Autoantikörpernachweis mittels indirekter Immunfluoreszenz an HEp-2-Zellen1)/Autoantibody detection using indirect immunofluorescence on HEp-2 cells
نویسندگان
چکیده
منابع مشابه
Autoantibody detection using indirect immunofluorescence on HEp-2 cells.
The detection of autoantibodies is an important element in the diagnosis and monitoring of disease progression in patients with autoimmune diseases. In laboratory diagnostic tests for connective tissue and autoimmune liver diseases, indirect immunofluorescence on HEp-2 cells plays a central role in a multistage diagnostic process. Despite the high quality of diagnostics, findings at different l...
متن کاملAutomated Indirect Immunofluorescence Evaluation of Antinuclear Autoantibodies on HEp-2 Cells
Indirect immunofluorescence (IIF) on human epithelial (HEp-2) cells is considered as the gold standard screening method for the detection of antinuclear autoantibodies (ANA). However, in terms of automation and standardization, it has not been able to keep pace with most other analytical techniques used in diagnostic laboratories. Although there are already some automation solutions for IIF inc...
متن کاملRoutine immunofluorescence detection of Ro/SS-A autoantibody using HEp-2 cells transfected with human 60 kDa Ro/SS-A.
BACKGROUND Ro/SS-A autoantibodies associated with systemic lupus erythematosus (SLE) and Sjögren syndrome may be missed during routine screening for antinuclear autoantibodies (ANA) by immunofluorescence using HEp-2 cells. AIMS To investigate the use of HEp-2 cells transfected with human 60 kDa Ro/SS-A for routine detection of these antibodies. METHODS 10,500 sera were screened at a dilutio...
متن کاملBenchmarking HEp-2 Cells Classification Methods
In this paper we report on the first edit ion of the HEp-2 Cells Classificat ion contest , held at the 2012 edit ion of the Internat ional Conference on Pattern Recognit ion, and focused on Indirect Immunofluorescence (IIF) image analysis. The IIF methodology is used to detect autoimmune diseases by searching for ant ibodies in the pat ient serum but, unfortunately, it is st ill a subject ive m...
متن کاملAnti-nuclear antibody screening using HEp-2 cells.
The American College of Rheumatology position statement on ANA testing stipulates the use of IIF as the gold standard method for ANA screening(1). Although IIF is an excellent screening test in expert hands, the technical difficulties of processing and reading IIF slides--such as the labor intensive slide processing, manual reading, the need for experienced, trained technologists and the use of...
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ژورنال
عنوان ژورنال: Laboratoriumsmedizin
سال: 2012
ISSN: 1439-0477,0342-3026
DOI: 10.1515/labmed-2011-0016